Coding

Part:BBa_K1349007:Design

Designed by: Alexia Satouf, Clara Bouyx, Aimeric Agaoua, Lambert Antoni, Vincent Castel   Group: iGEM14_Aix-Marseille   (2014-10-06)


cusR-lza


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 124
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 736


Design Notes

This part is a chimera containing the CusR response regulator fused to protein module designed as part of a protein-protein interaction system developped by Whitaker et al. The CusR response regulator when phosphorylated is expected to bind to the parts BBa_K1349002 and BBa_K1349003 acting as a negative or positive regulator of transcription. The LZA domain is a leucine zipper domain that is designed to form a complex with the leucine zipper domain od part BBa_K1349008.

This part is expected to bind the the CusR binding motif in parts BBa_K1349002 and BBa_K1349003 especially when phosphorylated, and so in the correct context repress or induce gene expression. The part is also expected to interact/bind with the part BBa_K1349008.

Source

Amplified from pWW2021,Whitaker et al. PNAS 2012

References

Whitaker WR, Davis SA, Arkin AP, Dueber JE. 2012. Engineering robust control of two-component system phosphotransfer using modular scaffolds. Proc Natl Acad Sci U S A. 109(44):18090-5. doi: 10.1073/pnas.1209230109.